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HAC – High-throughput Analysis Core
| Description | The HAC provides the research community access to state‐of‐the‐art proteomics instrumentation (Data Acquisition) and specialized analysis for high‐throughput data sets (Data Analysis). HAC Data Acquisition personnel and instrumentation are available to characterize proteins and peptides by mass spectrometry and high‐performance liquid chromatography, helping to identify patterns, trends or predictive factors from any large, complex data set. Core personnel assist with experimental design, perform sample preparation and run samples on one of the core’s mass spectrometers. Protein identification/quantitation results are stored in a relational database and are available for viewing and export. HAC Data Analysis Core personnel assist with or perform raw data analysis, data abstraction, and data integration on data sets acquired through HAC Data Acquisition or other means. Services include access to self‐guided data analysis via our online SPIRE proteomics data analysis workflow (http://proteinspire.org/SPIRE/startviews/welcome.jsf ) and more detailed analysis for specific questions or needs. If new computational algorithms and software are required for a project, Core personnel will provide software development and engineering. Commercial software packages are also available for routine analyses. Core personnel will manage data structures, data flow, and databases for data‐intensive projects. The Core will also assist with data mining and use of web‐based bioinformatics resources, including sequence analysis. |
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| Specialty equipment/services | The HAC is outfitted with an LTQ‐Velos Dual‐Pressure Linear Ion Trap mass spectrometer (MS) and an LCQ DECA XP Plus ion trap MS that are manufactured by Thermo Electron Corporation. An HP1100 Quaternary HPLC pump manufactured by Agilent Technologies, an Eksignent Nano 1D+ with an Eksigent autosampler, a FAMOS autosampler manufactured by LC Packings/Dionex, and a JANUS liquid handler manufactured by Perkin Elmer. The HAC maintains a high performance computing cluster of 300 processor cores, 500 gigabytes of RAM, and 48 terabytes of storage running Linux and utilizing cluster technologies such as Sun Grid Engine. |
| Web address(es) | HAC – High-throughput Analysis Core |
| Location |
Washington - Seattle Children's Hospital Seattle Children's Research Institute, 1900 Ninth Avenue, 9th Floor |
| Manager or contact | Eugene Kolker |
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This listing was last modified on February 19, 2010.
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