A Discussion of a High-throughput, Non-targeted Biochemical Profiling Platform and Case Studies (ITHS – Metabolomics Users Group Brown Bag Series)

There are many challenges associated with creating a functioning non-targeted biochemical profiling platform.  Current methods are often very time consuming and result in many false leads.  In the first part of the presentation I will discuss Metabolon’s analytical platform; a non-targeted, analytical metabolomics method which utilizes GC/MS and UPLC/MS/MS2 and a in-house generated spectral library of over 1500 compounds.  This platform permits rapid project completion, broad metabolome coverage, high confidence identifications, and does so with low process variability and a low false discovery rate.  For the rest of the presentation I will share some case studies from projects we have completed using this biochemical profiling technology, specifically on the discovery of a drug’s mechanism of action and biomarker discovery of prostate cancer aggressivity.