Tetramer Core Laboratory, Benaroya Research Institute
Tetramer Core Laboratory, Benaroya Research Institute, 1201 9th Ave, Seattle, WA 98101
1201 9th Avenue
Seattle
Washington
98101
US
The Tetramer Core Laboratory provides MHC class II tetramer reagents for collaborators both within and outside BRI. These tetramers are synthetic protein conjugates that allow the direct detection of antigen specific T cells by flow cytometry. Tetramers produced in our facility have been used to detect CD4+ (helper) T cells and have facilitated the study of viral immunity, autoimmune diseases, allergic responses, and cancer. The class II MHC proteins are produced using stable insect cell cultures and purified by affinity chromatography. The purified proteins are loaded with exogenous peptide and conjugated using PE labeled streptavidin. In some cases we are able to provide T cell clones for use as experimental positive controls.
Center Contact
Eddie James
Available to
Anyone
Resource Type
Core Laboratory
Institution
Benaroya Research Institute at Virginia Mason
Molecular Engineering & Sciences Building 3946 W Stevens Way NE Seattle, WA 98195
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(206) 744-2238 or (206) 744-3785(206) 744-2238 or (206) 744-3785
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908 Jefferson Street, Seattle, WA 98104, United States
(206) 543-7998(206) 543-7998
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Cell Function Analysis Core, Diabetes Research Center, 1660 S Columbian Way, Seattle, WA 98108
VA Laboratory, Building 13, VA Puget Sound Health Care System, 1660 S. Colombian Way, Seattle, WA 98108
Gene and Cell Therapy Lab, University of Washington Medical Center, 1959 NE Pacific St, Seattle, WA, 98195
(206) 616-4121(206) 616-4121
Department of Physics, Montana State University, Bozeman, MT 59717
P.O. Box 19024, DE-550 Seattle, WA 98109
CHDD, University of Washington, Box 357920, Seattle, WA 98195
(206) 543-7701(206) 543-7701
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(206) 732-1200(206) 732-1200
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Department of Laboratory Medicine, University of Washington, 1959 NE Pacific St, Seattle, WA 98195
Macromolecular Analysis Core, Physical Sciences, 108 University of Wyoming, 1000 E. University Ave, Laramie, WY 82071
MicroScale Life Sciences Center, University of Washington, Seattle, WA
Molecular Biology and Genomics, Center for Reproductive Biology, Washington State University, Pullman, WA 99164
UW Creative, 1705 NE Pacific St, Seattle, WA 98195 3 mi
UW Department of Engineering, William H. Foege Building, 3720 15th Ave NE, Seattle, WA 98195 3.01 mi
Applied Biomechanics Laboratory, University of Washington, 205 NE Northlake Way, Suite 100, Seattle, WA 98105 3.01 mi
3646 W Stevens Way NE, Seattle, WA 98195, United States 3.07 mi
Washington Nanofabrication Facility, University of Washington Botanic Gardens, 215 Fluke Hall, 4000 Mason Road, Seattle, WA 98195 3.08 mi
eScience Institute, University of Washington Physics/Astronomy Tower, 3910 15th Ave NE, Seattle, WA 98195 3.1 mi
Physics Instrument (Machine) Shop, University of Washington Department of Physics, 3910 15th Ave. NE, Seattle, WA 98195 3.1 mi
Center for Environmental Genomics, University of Washington, 616 NE Northlake Place, Seattle, WA 98195 3.15 mi
Center for Process Analysis & Control, 616 NE Northlake Place, Seattle, WA 98105 3.15 mi
Molecular Analysis Facility, University of Washington MolES, 3946 W Stevens Way NE, Seattle, WA 98195 3.16 mi
Molecular Engineering & Sciences Building 3946 W Stevens Way NE Seattle, WA 98195 3.16 mi
Applied Physics Laboratory University of Washington 1013 NE 40th Street Seattle WA 98105 3.17 mi
University of Washington, 1410 NE Campus Parkway, Seattle, WA 98195 3.31 mi
(206) 543-2100(206) 543-2100
Department of Environmental and Occupational Health Sciences, 4225 Roosevelt Way NE, Suite 100, Seattle, WA 98105 3.37 mi
Department of Environmental and Occupational Health Sciences, 4225 Roosevelt Way NE, Suite 100, Seattle, WA 98105 3.37 mi
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Chelan Ln, Seattle, WA 98105, United States 3.46 mi
Raitt Hall (RAI), Chelan Lane, Seattle, WA 98105, United States 3.47 mi
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The Institute is supported by grants UL1 TR002319, KL2 TR002317, and TL1 TR002318 from the NIH National Center for Advancing Translational Sciences through the Clinical and Translational Science Awards Program (CTSA).
Please help us continue to support your research by citing our grant number(s) in publications we supported.