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Tetramer Core Laboratory, Benaroya Research Institute

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Tetramer Core Laboratory, Benaroya Research Institute, 1201 9th Ave, Seattle, WA 98101
1201 9th Avenue Seattle Washington 98101 US
(206) 625-7373 x68176(206) 625-7373 x68176

The Tetramer Core Laboratory provides MHC class II tetramer reagents for collaborators both within and outside BRI. These tetramers are synthetic protein conjugates that allow the direct detection of antigen specific T cells by flow cytometry. Tetramers produced in our facility have been used to detect CD4+ (helper) T cells and have facilitated the study of viral immunity, autoimmune diseases, allergic responses, and cancer. The class II MHC proteins are produced using stable insect cell cultures and purified by affinity chromatography. The purified proteins are loaded with exogenous peptide and conjugated using PE labeled streptavidin. In some cases we are able to provide T cell clones for use as experimental positive controls.

Center Contact
Eddie James
Available to
Anyone
Resource Type
Core Laboratory
Institution
Benaroya Research Institute at Virginia Mason
Institution
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(206) 667-5313(206) 667-5313
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Cite ITHSThe Institute is supported by grants UL1 TR002319, KL2 TR002317, and TL1 TR002318 from the NIH National Center for Advancing Translational Sciences through the Clinical and Translational Science Awards Program (CTSA).

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